pET-30a(+)-Syk-tSH2
(Plasmid #111269)

• Purpose: express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)
• Depositing Lab: Carol Post
• Publication: Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5807-18. doi: 10.1039/c5cp05417f.

Backbone

• Vector backbone: pET-30a(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5422
• Total vector size (bp): 6015
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form
• Alt name: Spleen tyrosine kinase
• Species: M. musculus (mouse)
• Insert Size (bp): 786
• GenBank ID: NP_035648
• Entrez Gene: Syk (a.k.a. Sykb)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde I (unknown if destroyed)
• 3′ cloning site: Xho I (unknown if destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

encoding the following protein sequence: MSANHLTYFFGNITREEAEDYLVQGGMTDGLYLLRQSRNYLGGFALSVAHNRKAHHYTIERELNGTYAISGGRAHASPADLCHYHSQEPDGLICLLKKPFNRPPGVQPKTGPFEDLKENLIREYVKQTWNLQGQALEQAIISQKPQLEKLIATTAHEKMPWFHGNISRDESEQTVLIGSKTNGKFLIRARDNSGSYALCLLHEGKVLHYRIDRDKTGKLSIPEGKKFDTLWQLVEHYSYKPDGLLRVLTVPCQKIGAQ