pUBC_HA_PP7-mVenus
(Plasmid #111268)

• Purpose: Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag
• Depositing Lab: John Rinn
• Publication: Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10.1038/s41594-017-0015-3. doi: 10.1038/s41594-017-0015-3.

Backbone

• Vector backbone: pNEB193
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PP7-mVenus
• Species: Synthetic
• Mutation: PP7: C68S, S69G [delta]70-75 (non-aggregating)
• Promoter: hUBC
• Tags / Fusion Proteins:
  • mVenus (C terminal on insert)
  • HA (N terminal on insert)
  • SV40 NLS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry