pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2
(Plasmid #111259)

• Purpose: mini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 site
• Depositing Lab: Rosemarie Wilton
• Publication: Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi: 10.3389/fpls.2017.02242. eCollection 2017.

Backbone

• Vector backbone: pUC18T-mini-Tn7T-Gm
• Backbone manufacturer: Herbert Schweizer
• Total vector size (bp): 5859
• Vector type: Bacterial Expression
• Selectable markers: Ampicillin

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mTurquoise2
• Species: Synthetic
• Mutation: mTurquoise2 is codon optimized for expression in P. fluorescens
• Promoter: Pc

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pUC18T-mini-Tn7T-Gm_Pc_mTurquoise2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene NGS results identified a 963T>A mutation in first FRT site compared to the depositing lab's sequence. The effect of this variant on FLP recombination efficiency is unknown.