pSW002-Pc-TorA(sp)-mTurquoise2
(Plasmid #111254)

• Purpose: Broad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasm
• Depositing Lab: Rosemarie Wilton
• Publication: Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi: 10.3389/fpls.2017.02242. eCollection 2017.

Backbone

• Vector backbone: pME6031
• Backbone manufacturer: Dieter Haas; obtained from Culture Collection of Switzerland
• Total vector size (bp): 9352
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TorA(sp)-mTurquoise2
• Species: Synthetic
• Mutation: mTurquoise2 is codon optimized for expression in P. fluorescens
• Promoter: Pc

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pSW002_Pc_TorA(sp)_mTurquoise2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry