pSW002
(Plasmid
#111248)
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Purpose(Empty Backbone) Broad host-range bacterial vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111248 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepME6031
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Backbone manufacturerDieter Haas; obtained from Culture Collection of Switzerland
- Backbone size (bp) 8248
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Modifications to backboneSeveral restriction sites were removed to facilitate cloning
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSW002 was a gift from Rosemarie Wilton (Addgene plasmid # 111248 ; http://n2t.net/addgene:111248 ; RRID:Addgene_111248) -
For your References section:
A New Suite of Plasmid Vectors for Fluorescence-Based Imaging of Root Colonizing Pseudomonads. Wilton R, Ahrendt AJ, Shinde S, Sholto-Douglas DJ, Johnson JL, Brennan MB, Kemner KM. Front Plant Sci. 2018 Feb 1;8:2242. doi: 10.3389/fpls.2017.02242. eCollection 2017. 10.3389/fpls.2017.02242 PubMed 29449848