pBTM116-D9
(Plasmid
#111232)
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Purposeyeast expression vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111232 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBTM116-D9
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameccdB-cassette
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Speciesunknown
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Insert Size (bp)1873
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBTM116-D9 was a gift from Ulrich Stelzl (Addgene plasmid # 111232 ; http://n2t.net/addgene:111232 ; RRID:Addgene_111232) -
For your References section:
A stringent yeast two-hybrid matrix screening approach for protein-protein interaction discovery. Worseck JM, Grossmann A, Weimann M, Hegele A, Stelzl U. Methods Mol Biol. 2012;812:63-87. doi: 10.1007/978-1-61779-455-1_4. 10.1007/978-1-61779-455-1_4 PubMed 22218854
