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pSuperRetro-puro-RalB 45i
(Plasmid #11122)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PSuperRetroPuro
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RalAB siRNA
  • gRNA/shRNA sequence
    GACTATGAACCTACCAAAG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    30
  • Mutation
    N/A
  • Entrez Gene
    RALA (a.k.a. HINCONS, RAL)
  • Entrez Gene
    RALB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (unknown if destroyed)
  • 3′ cloning site Bgl II (unknown if destroyed)
  • 5′ sequencing primer pLXSN5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

RalB siRNA sequence is GACTATGAACCTACCAAAG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSuperRetro-puro-RalB 45i was a gift from Christopher Counter (Addgene plasmid # 11122 ; http://n2t.net/addgene:11122 ; RRID:Addgene_11122)

  • For your References section:

    Activation of RalA is critical for Ras-induced tumorigenesis of human cells. Lim KH, Baines AT, Fiordalisi JJ, Shipitsin M, Feig LA, Cox AD, Der CJ, Counter CM. Cancer Cell. 2005 Jun . 7(6):533-45. 10.1016/j.ccr.2005.04.030 PubMed 15950903
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