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Addgene

ECYFP
(Plasmid #111204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ECFP-Xpress tag-GFP10C fusion

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    original GFP10C from Roger Tsien

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ECYFP was a gift from Johannes A. Schmid (Addgene plasmid # 111204 ; http://n2t.net/addgene:111204 ; RRID:Addgene_111204)

  • For your References section:

    Oligomerization of the human serotonin transporter and of the rat GABA transporter 1 visualized by fluorescence resonance energy transfer microscopy in living cells. Schmid JA, Scholze P, Kudlacek O, Freissmuth M, Singer EA, Sitte HH. J Biol Chem. 2001 Feb 9;276(6):3805-10. doi: 10.1074/jbc.M007357200. Epub 2000 Nov 8. 10.1074/jbc.M007357200 PubMed 11071889
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