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Addgene

IKK2-EYFP
(Plasmid #111196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IKKbeta
  • Alt name
    IKBKB
  • Species
    H. sapiens (human)
  • Entrez Gene
    IKBKB (a.k.a. IKK-2, IKK-beta, IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB)
  • Tag / Fusion Protein
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IKK2-EYFP was a gift from Johannes A. Schmid (Addgene plasmid # 111196 ; http://n2t.net/addgene:111196 ; RRID:Addgene_111196)

  • For your References section:

    Signaling molecules of the NF-kappa B pathway shuttle constitutively between cytoplasm and nucleus. Birbach A, Gold P, Binder BR, Hofer E, de Martin R, Schmid JA. J Biol Chem. 2002 Mar 29;277(13):10842-51. doi: 10.1074/jbc.M112475200. Epub 2002 Jan 18. 10.1074/jbc.M112475200 PubMed 11801607
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