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Addgene

pLIC-SM-Myc
(Plasmid #111189)

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Item Catalog # Description Quantity Price (USD)
Plasmid 111189 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pLIC
  • Backbone manufacturer
    Vern Carruthers
  • Backbone size (bp) 6000
  • Modifications to backbone
    Modified by Sheiner et al. (PMID: 22144892)
  • Vector type
    Toxoplasma gondii expression
  • Promoter Endogenous
  • Selectable markers
    Chloramphenicol
  • Tag / Fusion Protein
    • Spaghetti Monster Myc Tag (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Spaghetti monster Myc gene was created in Loren L. Looger's Lab (doi:10.1038/nmeth.3365)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIC-SM-Myc was a gift from Silvia Moreno (Addgene plasmid # 111189 ; http://n2t.net/addgene:111189 ; RRID:Addgene_111189)

  • For your References section:

    Tagging of Weakly Expressed Toxoplasma gondii Calcium-Related Genes with High-Affinity Tags. Hortua Triana MA, Marquez-Nogueras KM, Chang L, Stasic AJ, Li C, Spiegel KA, Sharma A, Li ZH, Moreno SNJ. J Eukaryot Microbiol. 2018 Apr 19. doi: 10.1111/jeu.12626. 10.1111/jeu.12626 PubMed 29672999
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