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Addgene

pBADZ-HisCre
(Plasmid #111187)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111187 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD22
  • Total vector size (bp) 6427
  • Modifications to backbone
    The ampicillin resistance marker of pBAD22 was inactivated by digestion. The zeocin resistance marker was ligated yielding pBADZ.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre recombinase
  • Tag / Fusion Protein
    • His tag (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The protocol for preparation of electro-competent cells of DH10MultiBacCre,which carry the plasmid of pBADZ HisCre and enable the production of Cre recombinase, is described in Fitzgerald et al. (2006).
http://www.nature.com/articles/nmeth983

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBADZ-HisCre was a gift from Tim Richmond (Addgene plasmid # 111187 ; http://n2t.net/addgene:111187 ; RRID:Addgene_111187)

  • For your References section:

    Baculovirus expression system for heterologous multiprotein complexes. Berger I, Fitzgerald DJ, Richmond TJ. Nat Biotechnol. 2004 Dec;22(12):1583-7. doi: 10.1038/nbt1036. Epub 2004 Nov 28. 10.1038/nbt1036 PubMed 15568020
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