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pET28b-ETV6-ETS
(Plasmid #111180)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone size w/o insert (bp) 5422
  • Total vector size (bp) 5750
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ETV6 ETS domain
  • Alt name
    TEL
  • Species
    H. sapiens (human), M. musculus (mouse), B. taurus (bovine)
  • Insert Size (bp)
    300
  • GenBank ID
    XP_011518909
  • Entrez Gene
    ETV6 (a.k.a. TEL, TEL/ABL, THC5)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7promoter
  • 3′ sequencing primer T7terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene encodes the DNA-binding domain of a human protein known as ETV6, whose gene we in turn obtained from the Harvard Plasmid Repository, so the sequence is already in the public domain. See https://www.ncbi.nlm.nih.gov/pubmed/28592487.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-ETV6-ETS was a gift from Gregory Poon (Addgene plasmid # 111180 ; http://n2t.net/addgene:111180 ; RRID:Addgene_111180)

  • For your References section:

    Electrostatic control of DNA intersegmental translocation by the ETS transcription factor ETV6. Vo T, Wang S, Poon GMK, Wilson WD. J Biol Chem. 2017 Aug 11;292(32):13187-13196. doi: 10.1074/jbc.M117.792887. Epub 2017 Jun 7. 10.1074/jbc.M117.792887 PubMed 28592487
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