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Addgene

RT3GEP
(Plasmid #111164)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111164 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIX
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    miR-E (miR-30 variant)
  • Alt name
    turboGFP-miR-E-PGK-Puro
  • gRNA/shRNA sequence
    Ren.713
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Promoter T3G

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RT3GEP was a gift from Johannes Zuber (Addgene plasmid # 111164 ; http://n2t.net/addgene:111164 ; RRID:Addgene_111164)

  • For your References section:

    An optimized microRNA backbone for effective single-copy RNAi. Fellmann C, Hoffmann T, Sridhar V, Hopfgartner B, Muhar M, Roth M, Lai DY, Barbosa IA, Kwon JS, Guan Y, Sinha N, Zuber J. Cell Rep. 2013 Dec 26;5(6):1704-13. doi: 10.1016/j.celrep.2013.11.020. Epub 2013 Dec 12. 10.1016/j.celrep.2013.11.020 PubMed 24332856
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