pGEMT-Pax7bait-T2a-Cherry-pA
(Plasmid #111154)

• Purpose: knock in bait for Axolotl Pax7 locus
• Depositing Lab: Elly Tanaka
• Publication: Fei et al Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):12501-12506. doi: 10.1073/pnas.1706855114. Epub 2017 Oct 31.

Backbone

• Vector backbone: pGEMT
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pax7bait-T2a-Cherry-pA
• Alt name: Axolotl Pax7 bait
• Species: Ambystoma mexicanum
• Insert Size (bp): 1344
• Mutation: wt

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry