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pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert
(Plasmid #111153)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111153 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEMT
  • Vector type
    Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAP43-Cherry-T2a-mERt-Cre-Ert
  • Alt name
    Axolotl Pax7 bait
  • Species
    Ambystoma mexicanum
  • Insert Size (bp)
    1344
  • Mutation
    wt

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert was a gift from Elly Tanaka (Addgene plasmid # 111153 ; http://n2t.net/addgene:111153 ; RRID:Addgene_111153)

  • For your References section:

    Efficient gene knockin in axolotl and its use to test the role of satellite cells in limb regeneration. Fei JF, Schuez M, Knapp D, Taniguchi Y, Drechsel DN, Tanaka EM. Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):12501-12506. doi: 10.1073/pnas.1706855114. Epub 2017 Oct 31. 10.1073/pnas.1706855114 PubMed 29087939
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