pCAG-Trex2-bpA-EF1BFP
(Plasmid #111144)

• Purpose: Expression of mouse Trex2 and BFP reporter in mammalian cells
• Depositing Lab: Ralf Kuehn
• Publication: Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi: 10.1007/978-1-4939-9170-9_10.

Backbone

• Vector backbone: Bluescript
• Total vector size (bp): 7175
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mouse Trex2 coding region
• Alt name: Trex2
• Alt name: three prime repair exonuclease 2
• Species: M. musculus (mouse)
• Insert Size (bp): 711
• GenBank ID: NM_011907
• Entrez Gene: Trex2
• Promoter: CAG

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: M13 forward

Gene/Insert 2

• Gene/Insert name: BFP
• Insert Size (bp): 720
• Entrez Gene: Rnf112 (a.k.a. ZNF179, Zfp179, bfp, neurolastin)
• Promoter: EF1

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: AsiSI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: M13 forward

Resource Information

• Supplemental Documents:
  • pCAG-mTrex2-bpA-EF1BFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry