pCFD3-ovoD1
(Plasmid #111142)

• Purpose: Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster
• Depositing Lab: Norbert Perrimon
• Publication: Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-2756. doi: 10.1534/g3.118.200498.

Backbone

• Vector backbone: pCFD3
• Backbone size w/o insert (bp): 6230
• Total vector size (bp): 6250
• Vector type: Insect Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pCFD3-ovoD1
• gRNA/shRNA sequence: AAAAAGAGATGCCCGCAGAG
• Species: D. melanogaster (fly)
• Entrez Gene: ovo (a.k.a. Dmel_CG6824, CG15467, CG6824, Dmel\CG6824, Fs(1)K1103, Fs(1)K1237, Fs(1)K155, OVO, Ovo, Ovo-D, Shv, Svb, Svb/Ovo, fs(1)K1237, fs(1)M1, fs(1)M38, ovo/shavenbaby, ovo/svb, ovoD, svb, svb/ovo)
• Promoter: U6:3

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: TCGAAAATTTTTGAATAGCCCAGGT

Resource Information

• Supplemental Documents:
  • pCFD3-ovoD1.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry