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Addgene

pBW106
(Plasmid #111138)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111138 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS416gal
  • Backbone manufacturer
    Martin Funk
  • Backbone size w/o insert (bp) 5584
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MSI-ratAANAT-3FLAG (Leu2 deletion)
  • Alt name
    Aanat
  • Alt name
    aralkylamine N-acetyltransferase
  • Species
    R. norvegicus (rat)
  • Mutation
    Leu2 deletion
  • Entrez Gene
    Aanat (a.k.a. AA-NAT, Nat4)
  • Promoter Gal1
  • Tag / Fusion Protein
    • 3x Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer M13F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBW106 was a gift from Alexander Varshavsky (Addgene plasmid # 111138 ; http://n2t.net/addgene:111138 ; RRID:Addgene_111138)

  • For your References section:

    Degradation of Serotonin N-Acetyltransferase, a Circadian Regulator, by the N-end Rule Pathway. Wadas B, Borjigin J, Huang Z, Oh JH, Hwang CS, Varshavsky A. J Biol Chem. 2016 Aug 12;291(33):17178-96. doi: 10.1074/jbc.M116.734640. Epub 2016 Jun 23. 10.1074/jbc.M116.734640 PubMed 27339900
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