Rlf 328E-CAAX in pBabepuro
(Plasmid
#11111)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11111 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepbabepuro
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRlf
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SpeciesM. musculus (mouse)
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Insert Size (bp)1000
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Mutation328E CAAX deletion
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Entrez GeneRgl2 (a.k.a. KE1.5, Rab2l, Rgt2, Rlf)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR I (unknown if destroyed)
- 3′ cloning site EcoR I (unknown if destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rlf 328E-CAAX in pBabepuro was a gift from Christopher Counter (Addgene plasmid # 11111 ; http://n2t.net/addgene:11111 ; RRID:Addgene_11111) -
For your References section:
Activation of RalA is critical for Ras-induced tumorigenesis of human cells. Lim KH, Baines AT, Fiordalisi JJ, Shipitsin M, Feig LA, Cox AD, Der CJ, Counter CM. Cancer Cell. 2005 Jun . 7(6):533-45. 10.1016/j.ccr.2005.04.030 PubMed 15950903