pET-pro-siRNA-V2-TP53
(Plasmid
#111089)
-
PurposepET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 111089 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET-30a based pET-pro-siRNA-V2
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET-pro-siRNA-V2-TP53 was a gift from Linfeng HUANG (Addgene plasmid # 111089 ; http://n2t.net/addgene:111089 ; RRID:Addgene_111089) -
For your References section:
Milligram scale production of potent recombinant small interfering RNAs in Escherichia coli. Kaur G, Cheung HC, Xu W, Wong JV, Chan FF, Li Y, McReynolds L, Huang L. Biotechnol Bioeng. 2018 Jun 5. doi: 10.1002/bit.26740. 10.1002/bit.26740 PubMed 29873060