pET-pro-siRNA-V2
(Plasmid
#111086)
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Purpose(Empty Backbone) For production of recombinant siRNAs (pro-siRNAs) in E. coli.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111086 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-30a
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Vector typeBacterial Expression, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-pro-siRNA-V2 was a gift from Linfeng HUANG (Addgene plasmid # 111086 ; http://n2t.net/addgene:111086 ; RRID:Addgene_111086) -
For your References section:
Milligram scale production of potent recombinant small interfering RNAs in Escherichia coli. Kaur G, Cheung HC, Xu W, Wong JV, Chan FF, Li Y, McReynolds L, Huang L. Biotechnol Bioeng. 2018 Jun 5. doi: 10.1002/bit.26740. 10.1002/bit.26740 PubMed 29873060