AByF
(Plasmid #111084)

• Purpose: Integrates marker gene onto D. melanogaster Y chromosome
• Depositing Lab: Omar Akbari
• Publication: Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.1111/imb.12528.

Backbone

• Vector backbone: piggybac
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 8687
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Y homology arm left
• Species: D. melanogaster (fly)
• Insert Size (bp): 999

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ggaagcttcatgggatctgctg

Gene/Insert 2

• Gene/Insert name: gypsy insulator
• Species: D. melanogaster (fly)
• Insert Size (bp): 432

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGTAATAAGTGTGCGTTGAATTTATT

Gene/Insert 3

• Gene/Insert name: 3xP3 promoter
• Species: Synthetic
• Insert Size (bp): 264

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AATTCGAGCTCGCCCGGG

Gene/Insert 4

• Gene/Insert name: tdTomato
• Species: Synthetic
• Insert Size (bp): 1432

Cloning Information for Gene/Insert 4

• Cloning method: Gibson Cloning
• 5′ sequencing primer: atggtgagcaagggcgaggag

Gene/Insert 5

• Gene/Insert name: SV40 3'UTR
• Species: Synthetic
• Insert Size (bp): 248

Cloning Information for Gene/Insert 5

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCGCGACTCTAGATCATAA

Gene/Insert 6

• Gene/Insert name: CTCF insulator
• Species: D. melanogaster (fly)
• Insert Size (bp): 308

Cloning Information for Gene/Insert 6

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ccttgcagcgccacctgg

Gene/Insert 7

• Gene/Insert name: Y homology arm right
• Species: D. melanogaster (fly)
• Insert Size (bp): 964

Cloning Information for Gene/Insert 7

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TACAATTTGATTCAACGGTAGTGTTTTGT

Resource Information

• Supplemental Documents:
  • H-pBac-Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right-7.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry