GFP-VIVIT
(Plasmid #11106)

• Depositing Lab: Anjana Rao
• Publication: Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VIVIT
• Alt name: NFAT regulatory domain
• Insert Size (bp): 51
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bsp120I (destroyed during cloning)
• 3′ cloning site: NcoI (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Oligo coding for MAGPHPVIVITGPHEE at the N-terminus of GFP. GPPVAS spacer sequence before GFP.