mTalin2_R13-DD
(Plasmid
#111047)
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PurposePlasmid expresses the talin2 rod domain R13 and the dimerisation domian DD.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111047 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET151TOPO
- Backbone size w/o insert (bp) 5362
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTLN2
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SpeciesM. musculus (mouse)
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Insert Size (bp)828
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Entrez GeneTln2 (a.k.a. 5730421P04Rik, mKIAA0320)
- Promoter T7
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Tag
/ Fusion Protein
- His Tag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mTalin2_R13-DD was a gift from Ben Goult (Addgene plasmid # 111047 ; http://n2t.net/addgene:111047 ; RRID:Addgene_111047) -
For your References section:
The tale of two talins - two isoforms to fine-tune integrin signalling. Gough RE, Goult BT. FEBS Lett. 2018 May 3. doi: 10.1002/1873-3468.13081. 10.1002/1873-3468.13081 PubMed 29723415