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Addgene

CA-NFAT2
(Plasmid #11102)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11102 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    GFP-RV-DV
  • Backbone manufacturer
    Genetics Institute
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFAT2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2100
  • Mutation
    Constitutively active: multiple serine to alanine mutations in the SRR and SPXX repeat motifs in the NFAT2 regulatory domain
  • Entrez Gene
    Nfatc1 (a.k.a. 2210017P03Rik, NF-ATc, NFAT2, NFATc, Nfatcb)
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • GFP

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CA-NFAT2 was a gift from Anjana Rao (Addgene plasmid # 11102 ; http://n2t.net/addgene:11102 ; RRID:Addgene_11102)
  • For your References section:

    NFAT1 and NFAT2 are positive regulators of IL-4 gene transcription. Monticelli S, Rao A. Eur J Immunol. 2002 Oct . 32(10):2971-8. 10.1002/1521-4141(2002010)32:10<2971::AID-IMMU2971>3.0.CO;2-G PubMed 12355451