CPN60-COMP-blac-flag-his
(Plasmid #111004)

• Purpose: Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
• Depositing Lab: Gavin Wright
• Publication: Knockel et al Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. Epub 2021 Jan 27.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
• Backbone size w/o insert (bp): 7661
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CPN60
• Alt name: PF3D7_1232100
• Species: Synthetic
• Insert Size (bp): 2085
• Mutation: All NXT/S sites mutated to NXA, where X is any amino acid except Proline
• Entrez Gene: PF3D7_1232100 (a.k.a. PF3D7_1232100, PFL1545c)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Exogenous signal peptide of mouse origin (N terminal on insert)
  • rat Cd4d3+4, beta-lactamase, triple FLAG tag, 6xHis tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: Asc1 (not destroyed)

Resource Information

• Supplemental Documents:
  • E3_CPN60.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry