PSOP7-COMP-blac-flag-his
(Plasmid
#110996)
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PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110996 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTT3
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Backbone manufacturerpTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
- Backbone size w/o insert (bp) 7661
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesecreted ookinete protein, putative (PSOP7)
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Alt namePF3D7_1340000
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SpeciesSynthetic
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Insert Size (bp)1854
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MutationAll NXT/S sites mutated to NXA, where X is any amino acid except Proline
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Entrez GenePF3D7_1340000 (a.k.a. PF3D7_1340000, MAL13P1.203)
- Promoter CMV
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Tags
/ Fusion Proteins
- Exogenous signal peptide of mouse origin (N terminal on insert)
- rat Cd4d3+4, beta-lactamase, triple FLAG tag, 6xHis tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Not1 (not destroyed)
- 3′ cloning site Asc1 (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PSOP7-COMP-blac-flag-his was a gift from Gavin Wright (Addgene plasmid # 110996 ; http://n2t.net/addgene:110996 ; RRID:Addgene_110996) -
For your References section:
Systematic Identification of Plasmodium Falciparum Sporozoite Membrane Protein Interactions Reveals an Essential Role for the p24 Complex in Host Infection. Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, Sauerwein RW, Rayner JC, Billker O, Wright GJ. Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. Epub 2021 Jan 27. 10.1074/mcp.RA120.002432 PubMed 33515807