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GAP50-COMP-blac-flag-his
(Plasmid #110956)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110956 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
  • Backbone size w/o insert (bp) 7661
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    secreted acid phosphatase (GAP50)
  • Alt name
    PF3D7_0918000
  • Alt name
    PfGAP50, PfSAP, SAP
  • Species
    Synthetic
  • Insert Size (bp)
    1188
  • Mutation
    All NXT/S sites mutated to NXA, where X is any amino acid except Proline
  • Entrez Gene
    PF3D7_0918000 (a.k.a. PF3D7_0918000, PFI0880c)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Exogenous signal peptide of mouse origin (N terminal on insert)
    • rat Cd4d3+4, beta-lactamase, triple FLAG tag, 6xHis tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site Asc1 (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GAP50-COMP-blac-flag-his was a gift from Gavin Wright (Addgene plasmid # 110956 ; http://n2t.net/addgene:110956 ; RRID:Addgene_110956)

  • For your References section:

    Systematic Identification of Plasmodium Falciparum Sporozoite Membrane Protein Interactions Reveals an Essential Role for the p24 Complex in Host Infection. Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, Sauerwein RW, Rayner JC, Billker O, Wright GJ. Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. Epub 2021 Jan 27. 10.1074/mcp.RA120.002432 PubMed 33515807
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