pANY1
(Plasmid #110949)

• Purpose: Universal mini-vector for efficient cloning of DNA and expression of protein in E. coli.
• Depositing Lab: Yingfeng An
• Publication: Liu et al Biochem Biophys Res Commun. 2018 Mar 18;497(4):978-982. doi: 10.1016/j.bbrc.2018.02.099. Epub 2018 Feb 12.

Backbone

• Vector backbone: pANY1
• Backbone manufacturer: Yingfeng An
• Total vector size (bp): 2663
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ccd B cassette
• Species: Synthetic
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Supplemental Documents:
  • pANY1.gb
  • pANY1 supporting documentation

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

During molecular cloning, DNA of interest can be inserted into pANY1 using both MCS-1 and MCS-2, with deletion of the ccdB cassette. Thus the recombinant plasmid can be transformed into commonly used E. coli strains (e.g., JM109, BL21, DH5a). Because the original plasmid, pANY1, cannot be transformed into these E. coli strains, false positives can be eliminated.