pmTurquoise2-LAMP1
(Plasmid #110948)

• Purpose: Expresses LAMP1 fused to mTurquoise2
• Depositing Lab: Pablo Rivera-Fuentes
• Publication: Halabi et al Nat Commun. 2019 Mar 15;10(1):1232. doi: 10.1038/s41467-019-09217-7.

Backbone

• Vector backbone: pAc5.1/V5-His B
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7459
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lysosomal-associated membrane protein 1
• Alt name: LAMP1
• Species: H. sapiens (human)
• Insert Size (bp): 2179
• Entrez Gene: LAMP1 (a.k.a. CD107a, LAMPA, LGP120)
• Promoter: CMV
• Tag / Fusion Protein:
  • mTurquoise2 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV

Resource Information

• Supplemental Documents:
  • pLAMP1-mTurquoise2-assembled.gb
• A portion of this plasmid was derived from a plasmid made by: This plasmid was assembled using fragments from plasmids #16290 (backbone) and #36204 (mTurquoise2 insert).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry