pmTurquoise2-LC3
(Plasmid #110947)

• Purpose: Expresses the autophagosome marker LC3 fused to mTurquoise2
• Depositing Lab: Pablo Rivera-Fuentes
• Publication: Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681-2687. doi: 10.1039/d0ob02259d. Epub 2021 Feb 26.

Backbone

• Vector backbone: pEGFP-C2
• Backbone size w/o insert (bp): 1048
• Total vector size (bp): 5093
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Microtubule-associated proteins 1A/1B light chain 3B
• Alt name: LC3
• Species: H. sapiens (human)
• Entrez Gene: MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
• Tag / Fusion Protein:
  • mTurquoise2

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: SV40

Resource Information

• Supplemental Documents:
  • pmTurquoise2-LC3-assembled.gb
• A portion of this plasmid was derived from a plasmid made by: This plasmid was assembled using fragments from plasmids #24920 (backbone) and #36204 (mTurquoise2 insert).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry