pmTurquoise2-Drp1
(Plasmid #110946)

• Purpose: Expresses dynamin-like protein 1 fused to mTurquoise2
• Depositing Lab: Pablo Rivera-Fuentes
• Publication: mTurquoise2-labeled markers of mitophagy (unpublished)

Backbone

• Vector backbone: mCh-Drp1 (Plasmid #49152)
• Backbone manufacturer: Gia Voeltz lab
• Backbone size w/o insert (bp): 3833
• Total vector size (bp): 6792
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dynamin-1-like protein
• Alt name: Drp1
• Species: H. sapiens (human)
• Insert Size (bp): 2959
• Entrez Gene: DNM1L (a.k.a. DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5)
• Promoter: CMV
• Tag / Fusion Protein:
  • mTurquoise2

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV

Resource Information

• Supplemental Documents:
  • pmTurquoise-Drp1-assembled.gb
• A portion of this plasmid was derived from a plasmid made by: This plasmid was assembled using fragments from plasmids #49152 (backbone) and #36204 (mTurquoise2 insert).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry