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pLenti6.3-OGDH_wobble
(Plasmid #110938)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110938 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6.3/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 9387
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OGDH wobble cDNA
  • Alt name
    KGDH
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3071
  • Mutation
    15 silent wobble mutations corresponding to three shRNA sites
  • GenBank ID
    NM_002541.3
  • Entrez Gene
    OGDH (a.k.a. AKGDH, E1k, E1o, KGD1, OGDC, OGDH-E1, OGDH2, OGDHD)
  • Promoter SV40

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6.3-OGDH_wobble was a gift from William Hahn (Addgene plasmid # 110938 ; http://n2t.net/addgene:110938 ; RRID:Addgene_110938)

  • For your References section:

    PIK3CA mutant tumors depend on oxoglutarate dehydrogenase. Ilic N, Birsoy K, Aguirre AJ, Kory N, Pacold ME, Singh S, Moody SE, DeAngelo JD, Spardy NA, Freinkman E, Weir BA, Tsherniak A, Cowley GS, Root DE, Asara JM, Vazquez F, Widlund HR, Sabatini DM, Hahn WC. Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):E3434-E3443. doi: 10.1073/pnas.1617922114. Epub 2017 Apr 10. 10.1073/pnas.1617922114 PubMed 28396387
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