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p139 pETF-GST RanBP10 L301I
(Plasmid #11092)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11092 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETF-GST
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RanBP10
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1250
  • Mutation
    L301I; see depositor comments below
  • Entrez Gene
    Ranbp10 (a.k.a. 4432417N03Rik)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The closest NCBI protein match is Q6VN19.2. Please note that this construct contains amino acids 1-407 and is thus a truncated form.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p139 pETF-GST RanBP10 L301I was a gift from Ramesh Shivdasani (Addgene plasmid # 11092 ; http://n2t.net/addgene:11092 ; RRID:Addgene_11092)
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