BSP188
(Plasmid
#110917)
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PurposeFor cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110917 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBSP179
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Backbone manufacturerMendenhall Lab
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Vector typeWorm Expression, Synthetic Biology ; Yeast Homologous Recombination cloning for Universal MosSCI
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameunc54 Terminator
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SpeciesC. elegans (nematode)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BSP188 was a gift from Alexander Mendenhall (Addgene plasmid # 110917 ; http://n2t.net/addgene:110917 ; RRID:Addgene_110917) -
For your References section:
A toolkit for DNA assembly, genome engineering and multicolor imaging for C. elegans. Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A. Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. Epub 2018 Apr 13. 10.1016/j.tma.2018.01.001 PubMed 33693300