KG#865
(Plasmid #110912)

• Purpose: Coding sequence for attaching the Auxin Inducible Degron to the C-terminus of a protein followed by a 5X Myc tag with spacers
• Depositing Lab: Kenneth Miller
• Publication: An Intron-Compatible Marker for Long Distance CRISPR-Mediated Gene Editing in Caenorhabditis elegans (unpublished)

Backbone

• Vector backbone: pUC
• Backbone size w/o insert (bp): 2710
• Total vector size (bp): 3136
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Auxin Inducible Degron-5X Myc-Spacers for C-terminal attachment
• Species: A. thaliana (mustard weed)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • Annotated ApE and Strider formatted file--download free ApE plasmid editor to view

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Submitted the Degron+5x Myc-Spacers sequence (see below) to Genscript for GenePlus Economy synthesis and cloning into the EcoRV site of pUC57. Use this plasmid for Gibson Assemblies of this sequence onto the C-terminus of a gene.