pQCXIX-Myc-hTIM3
(Plasmid #110893)

• Purpose: Expression of Myc-tagged hTIM3 at the cell surface
• Depositing Lab: Hyeryun Choe
• Publication: Jemielity et al PLoS Pathog. 2013 Mar;9(3):e1003232. doi: 10.1371/journal.ppat.1003232. Epub 2013 Mar 28.

Backbone

• Vector backbone: pQCXIX
• Backbone manufacturer: BD Biosciences
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Hepatitis A virus Cellular Receptor 2 (HAVCR2)
• Alt name: T-Cell Immunoglobulin and Mucin Domain 3 (TIM3)
• Species: H. sapiens (human)
• Insert Size (bp): 930
• Entrez Gene: HAVCR2 (a.k.a. CD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI or AgeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Best if grown with in the presence of chloramphenicol