pDEST-periHisMBP
(Plasmid #11086)

• Purpose: (Empty Backbone)
• Depositing Lab: David Waugh
• Publication: Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Backbone

• Vector backbone: pMal-C2
• Backbone manufacturer: New England Biolabs
• Backbone size (bp): 6000
• Vector type: Bacterial Expression
• Promoter: tac promoter
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • MBP (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: plasmid must be grown in gyrA mutant resistant to the toxin CcdB
• Copy number: Low Copy

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: MBP-F
• 3′ sequencing primer: M13_pUC_fwd; M13-F20

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The MBP ORF can be sequenced using 5'-GAG CGG ATA ACA ATT TCA CAC AGG-3' as a tac promoter sequencing primer (in the forward direction). To sequence inserts fused to the C-terminus of MBP, the primers are 5'-GAT GAA GCC CTG AAA GAC GCG CAG-3' (forward) and 5'-GCA AGG CGA TTA AGT TGG GTA ACG C-3' (reverse).