pCS2 you
(Plasmid
#11084)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11084 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemodified pCS2+
- Backbone size w/o insert (bp) 4100
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Vector typezebrafish
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameyou
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Alt namescube2
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)3400
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GenBank IDAY741664
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Entrez Genescube2 (a.k.a. wu:fc27f08, yo, you)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A modified version of pCS2+ was generated by cloning a 41bp fragment into its EcoRI and XbaI sites, abolishing the original sites and introducing XbaI, SacI, ApaI, PstI, and XhoI sequences in a 5' to 3' orientation with respect to the SP6 promoter.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2 you was a gift from William Talbot (Addgene plasmid # 11084 ; http://n2t.net/addgene:11084 ; RRID:Addgene_11084) -
For your References section:
The you gene encodes an EGF-CUB protein essential for Hedgehog signaling in zebrafish. Woods IG, Talbot WS. PLoS Biol. 2005 Mar . 3(3):e66. 10.1371/journal.pbio.0030066 PubMed 15660164