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Addgene

EFS-GFP
(Plasmid #110834)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110834 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRL.sin-18.ppt.TCF/LEF:GFP.pre
  • Modifications to backbone
    The 4 repeats of M67 at STAT3-GFP plasmid was deleted and only TATA box is present at the promoter region of GFP.
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    elongation factor 1alpha binding sequence (EFS) at GFP promoter region

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EFS-GFP was a gift from Michael Lewis (Addgene plasmid # 110834 ; http://n2t.net/addgene:110834 ; RRID:Addgene_110834)

  • For your References section:

    STAT3 signaling is activated preferentially in tumor-initiating cells in claudin-low models of human breast cancer. Wei W, Tweardy DJ, Zhang M, Zhang X, Landua J, Petrovic I, Bu W, Roarty K, Hilsenbeck SG, Rosen JM, Lewis MT. Stem Cells. 2014 Oct;32(10):2571-82. doi: 10.1002/stem.1752. 10.1002/stem.1752 PubMed 24891218
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