dCas9-KRAB Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid dCas9-KRAB with the following enzymes: NdeI and EcoRI-HF.
Expected Bands
NdeI: 6.6 kb & 0.9 kb
EcoRI-HF: 6.7 kb & 0.6 kb
EcoRI-HF/ NdeI: 3.3 kb, 2.7 kb, 0.9 kb & 0.6 kb
We observe the expected bands for this digest.
