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Purposetet-inducible NOS2 expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLIX_403
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Backbone manufacturerDavid Root (Addgene plasmid # 41395)
- Backbone size w/o insert (bp) 9396
- Total vector size (bp) 12857
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNOS2
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Alt namenitric oxide synthase 2
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SpeciesH. sapiens (human)
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Insert Size (bp)3461
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Entrez GeneNOS2 (a.k.a. HEP-NOS, INOS, NOS, NOS2A)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer LNCX (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLIX403-hNOS2 was a gift from Edward Morgan (Addgene plasmid # 110800 ; http://n2t.net/addgene:110800 ; RRID:Addgene_110800) -
For your References section:
Nitric oxide-regulated proteolysis of human CYP2B6 via the ubiquitin-proteasome system. Lee CM, Tripathi S, Morgan ET. Free Radic Biol Med. 2017 Jul;108:478-486. doi: 10.1016/j.freeradbiomed.2017.04.015. Epub 2017 Apr 17. 10.1016/j.freeradbiomed.2017.04.015 PubMed 28427998
