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pET-22b PA K397D/D425K
(Plasmid #11080)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 11080 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET-22b
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PA
  • Alt name
    protective antigen
  • Species
    Bacillus anthracis
  • Insert Size (bp)
    2200
  • Mutation
    K397D; D425K

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Double mutant. NdeI site at start of mature protein. Stop codon, out of frame His tag on vector, then another stop codon. There are 10 extra amino acids at the 5' end of the coding sequence corresponding to a second leader sequence. E285Q is the correct sequence as determined by crystallography and different from Genbank.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-22b PA K397D/D425K was a gift from John Collier (Addgene plasmid # 11080)
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