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Addgene

Human Parkin (1-465)
(Plasmid #110758)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110758 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Merck
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PRKN
  • Species
    H. sapiens (human)
  • Entrez Gene
    PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
  • Promoter T7
  • Tag / Fusion Protein
    • N-His6-GST-3C (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AACGTATTGAAGCTATCCC
  • 3′ sequencing primer GGGTTATGCTAGTTATTGC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Parkin gene obtained through gene synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Parkin (1-465) was a gift from David Komander (Addgene plasmid # 110758 ; http://n2t.net/addgene:110758 ; RRID:Addgene_110758)

  • For your References section:

    Structure of the human Parkin ligase domain in an autoinhibited state. Wauer T, Komander D. EMBO J. 2013 Jul 31;32(15):2099-112. doi: 10.1038/emboj.2013.125. Epub 2013 May 31. 10.1038/emboj.2013.125 PubMed 23727886
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