Human RNF144B (27-236)
(Plasmid
#110753)
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PurposeBacterial Expression of RNF144B RBR domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110753 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Backbone manufacturerMerck
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRNF144B
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SpeciesH. sapiens (human)
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Entrez GeneRNF144B (a.k.a. PIR2, IBRDC2, p53RFP, KIAA0161, MGC71786, bA528A10.3)
- Promoter T7
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Tag
/ Fusion Protein
- N-His6-GST-3C (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer AATACGACTCACTATAGGG
- 3′ sequencing primer GGGTTATGCTAGTTATTGC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byRNF144B: cDNA library (generated at LMB)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human RNF144B (27-236) was a gift from David Komander (Addgene plasmid # 110753 ; http://n2t.net/addgene:110753 ; RRID:Addgene_110753) -
For your References section:
Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked Ubiquitin Signaling. Michel MA, Swatek KN, Hospenthal MK, Komander D. Mol Cell. 2017 Oct 5;68(1):233-246.e5. doi: 10.1016/j.molcel.2017.08.020. Epub 2017 Sep 21. 10.1016/j.molcel.2017.08.020 PubMed 28943312