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Addgene

Pediculus humanus corporis PINK1 (115 - 575)
(Plasmid #110750)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110750 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Merck
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Phum_PHUM577390
  • Alt name
    PINK1
  • Species
    Pediculus humanus corporis
  • Entrez Gene
    Phum_PHUM577390 (a.k.a. Phum_PHUM577390)
  • Promoter T7
  • Tag / Fusion Protein
    • N-His6-GST-3C (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AATACGACTCACTATAGGG
  • 3′ sequencing primer GGGTTATGCTAGTTATTGC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PhPink1: obtained through gene synthesis
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pediculus humanus corporis PINK1 (115 - 575) was a gift from David Komander (Addgene plasmid # 110750 ; http://n2t.net/addgene:110750 ; RRID:Addgene_110750)

  • For your References section:

    Structure of PINK1 in complex with its substrate ubiquitin. Schubert AF, Gladkova C, Pardon E, Wagstaff JL, Freund SMV, Steyaert J, Maslen SL, Komander D. Nature. 2017 Dec 7;552(7683):51-56. doi: 10.1038/nature24645. Epub 2017 Oct 30. 10.1038/nature24645 PubMed 29160309
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