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Addgene

pET-15b LFN-DTA
(Plasmid #11075)

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Item Catalog # Description Quantity Price (USD)
Plasmid 11075 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LFN-DTA
  • Alt name
    LF
  • Alt name
    DT
  • Species
    Bacillus anthracis and Corynebacterium diphtheriae
  • Insert Size (bp)
    1400
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Chimeric protein composed of the PA recognition domain of LF (LFN; residues 1-255) fused to the catalytic chain of diptheria toxin (DTA). pET-15b LFN was digested with NdeI/NcoI and pSBXLIII (DTA containing) was digested with NcoI/BamHI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-15b LFN-DTA was a gift from John Collier (Addgene plasmid # 11075)

  • For your References section:

    Protective antigen-binding domain of anthrax lethal factor mediates translocation of a heterologous protein fused to its amino- or carboxy-terminus. Milne JC, Blanke SR, Hanna PC, Collier RJ. Mol Microbiol. 1995 Feb . 15(4):661-6. 10.1111/j.1365-2958.1995.tb02375.x PubMed 7783638
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