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Addgene

Human USP30 (64-502, construct 13i, C77A, MG-31-28)
(Plasmid #110747)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110747 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Merck
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    USP30
  • Species
    H. sapiens (human)
  • Mutation
    C77A, F348D, M350S, I353E + insertion deletion: 64-178; GSGS; 217-357; SNA; 432-502
  • Entrez Gene
    USP30
  • Promoter T7
  • Tag / Fusion Protein
    • N-His6-GST-3C (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AATACGACTCACTATAGGG
  • 3′ sequencing primer GGGTTATGCTAGTTATTGC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    USP30 gene obtained through gene synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human USP30 (64-502, construct 13i, C77A, MG-31-28) was a gift from David Komander (Addgene plasmid # 110747 ; http://n2t.net/addgene:110747 ; RRID:Addgene_110747)

  • For your References section:

    Mechanism and regulation of the Lys6-selective deubiquitinase USP30. Gersch M, Gladkova C, Schubert AF, Michel MA, Maslen S, Komander D. Nat Struct Mol Biol. 2017 Sep 25. doi: 10.1038/nsmb.3475. 10.1038/nsmb.3475 PubMed 28945249
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