pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2
(Plasmid
#110735)
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PurposeRecombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110735 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHDR
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH2B-mOrange2
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2 was a gift from Robert Judson-Torres (Addgene plasmid # 110735 ; http://n2t.net/addgene:110735 ; RRID:Addgene_110735) -
For your References section:
Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 Activation. Zeng H, Jorapur A, Shain AH, Lang UE, Torres R, Zhang Y, McNeal AS, Botton T, Lin J, Donne M, Bastian IN, Yu R, North JP, Pincus L, Ruben BS, Joseph NM, Yeh I, Bastian BC, Judson RL. Cancer Cell. 2018 Jul 9;34(1):56-68.e9. doi: 10.1016/j.ccell.2018.05.014. 10.1016/j.ccell.2018.05.014 PubMed 29990501