pStr-KDEL-IRES-ST-mCherry
(Plasmid #110727)

• Purpose: Expresses an Str-KDEL Hook for the RUSH system and separately Sialyltransferase-mCherry
• Depositing Lab: David Stephens
• Publication: McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.201806035. doi: 10.1083/jcb.201806035.

Backbone

• Vector backbone: pIRESneo3
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Str-KDEL
• Species: Synthetic
• Promoter: CMV
• Tag / Fusion Protein:
  • Str Streptavidin binding peptide (SBP) (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: IRES
• 3′ sequencing primer: mCherry-R

Gene/Insert 2

• Gene/Insert name: STase sialyltransferase
• Species: H. sapiens (human)
• Mutation: contains only amino acids 1 to 43 of ST
• Entrez Gene: ST6GAL1 (a.k.a. CDw75, SIAT1, ST6GalI, ST6N)
• Promoter: IRES
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: .
• 3′ sequencing primer: .

Resource Information

• Supplemental Documents:
  • Str-KDEL-IRES-ST-mCherry information
• A portion of this plasmid was derived from a plasmid made by: Franck Perez (Institut Curie) Str-KDEL_ST-SBP-mCherry (Plasmid #65265)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry